{"id":236,"date":"2023-06-27T13:47:03","date_gmt":"2023-06-27T13:47:03","guid":{"rendered":"https:\/\/ppsp.com.pk\/?p=236"},"modified":"2023-09-01T07:11:04","modified_gmt":"2023-09-01T07:11:04","slug":"the-makeup-of-a-plant","status":"publish","type":"post","link":"https:\/\/ppsp.com.pk\/?p=236","title":{"rendered":"Teak propagation"},"content":{"rendered":"<p><a href=\"https:\/\/www.researchgate.net\/publication\/225242053_An_efficient_method_for_clonal_propagation_and_in_vitro_establishment_of_softwood_shoots_from_epicormic_buds_of_teak_Tectona_grandis_L\">https:\/\/www.researchgate.net\/publication\/225242053_An_efficient_method_for_clonal_propagation_and_in_vitro_establishment_of_softwood_shoots_from_epicormic_buds_of_teak_Tectona_grandis_L<\/a><\/p>\n<div class=\"nova-legacy-o-stack__item\">\n<div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-none nova-legacy-e-text--color-inherit\">\n<div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-none nova-legacy-e-text--color-inherit\">Softwood shoots were produced from 40 cm long stem segments placed horizontally in flat trays containing sterilized sand under natural light or shade conditions for subsequent rooting and micropropagation studies in teak (Tectona grandis L.). Higher number of shoots (6.17) per log was produced under natural light as compared to shade conditions. Forcing was also better in natural light as compared to shade in terms of shoot length, number of nodes or leaves. For rooting, 2\u20134 cm long softwood shoots were excised and treated with either indole-3-butyric acid (IBA) or \u03b1-naphthyl acetic acid (NAA) at 0, 1000, 2000 or 3000 \u03bcmol\u00b7L\u22121 each or with combinations (1000 + 1000, 2000 + 2000 or 3000 + 3000 \u03bcmol\u00b7L\u22121) and then placed in flat trays containing autoclaved sand at 25 \u00b1 2\u00b0C in 16 h photoperiod at 35 \u03bcmol\u00b7m\u22122\u00b7s\u22121. After 28 days, softwood cuttings treated with IBA + NAA (3000 + 3000 \u03bcmol\u00b7L\u22121) had highest rooting percentage (89.3%) with 5.5 mean roots. Shoot apex and nodal explants of softwood cuttings were pretreated with 0.1% (w\/v) ascorbic acid, boric acid, activated charcoal, citric acid, glutamine or polyvinylpolypyrollidone (PVP) for 24 h to remove phenolic compounds before surface disinfestation. Glutamine (Gl) and PVP were equally effective resulting in 60% establishment of shoot apices on MS medium supplemented with 10 \u03bcmol\u00b7L\u22121 6-benzylaminopurine (BAP) + 5 \u03bcmol\u00b7L\u22121 NAA. Using shoot apices, highest (42.80) number of multiple shoots with 54.33 mm shoot length were obtained on MS + BAP (8.8 \u03bcmol\u00b7L\u22121) + IBA (2 \u03bcmol\u00b7L\u22121) after 45 days. Shoots were successfully rooted and acclimatized to greenhouse conditions.<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>https:\/\/www.researchgate.net\/publication\/225242053_An_efficient_method_for_clonal_propagation_and_in_vitro_establishment_of_softwood_shoots_from_epicormic_buds_of_teak_Tectona_grandis_L Softwood shoots were produced from 40 cm long stem segments placed horizontally in flat trays containing sterilized sand under natural light or shade conditions for subsequent rooting and micropropagation studies in teak (Tectona grandis L.). Higher number of shoots (6.17) per log was produced under natural light as compared to shade conditions. Forcing was [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":109,"comment_status":"open","ping_status":"open","sticky":false,"template":"elementor_canvas","format":"standard","meta":{"_eb_attr":"","_themeisle_gutenberg_block_has_review":false,"footnotes":""},"categories":[12],"tags":[],"class_list":["post-236","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-propagation"],"_links":{"self":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts\/236","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=236"}],"version-history":[{"count":9,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts\/236\/revisions"}],"predecessor-version":[{"id":2240,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts\/236\/revisions\/2240"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/media\/109"}],"wp:attachment":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=236"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=236"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}