{"id":2048,"date":"2023-07-17T14:33:37","date_gmt":"2023-07-17T14:33:37","guid":{"rendered":"https:\/\/ppsp.com.pk\/?p=2048"},"modified":"2026-08-02T04:12:31","modified_gmt":"2026-08-02T04:12:31","slug":"saving-the-deep-blue-sea","status":"publish","type":"post","link":"https:\/\/ppsp.com.pk\/?p=2048","title":{"rendered":"Saving the endangered flora of Physalis minima by using in vitro technology"},"content":{"rendered":"\r\n<p class=\"wp-block-paragraph\"><strong>Saving the endangered flora of Physalis minima by plant tissue culture\u00a0<\/strong><\/p>\r\n<p id=\"viewer-knak\">Physalis minima is a promising medicinal plant of the family Solanaceae, but its survival is under<br \/>threat, and limited long-term conservation approaches are available. We employed an in vitro plant tissue<br \/>culture technique to develop a mass propagation system for the effective multiplication and conservation of P.<br \/>minima. Juvenile leaves were cultured on MS basal medium supplemented with 0.1, 0.5, 1.0, 2.0, and 5.0 \u00b5M<br \/>thidiazuron (TDZ). As a result, 100% callus induction was achieved on the medium containing 1.0 \u00b5M TDZ.<br \/>Notably, calli grown on 1.0 \u00b5M TDZ exhibited greater potential for shoot regeneration and proliferation in<br \/>the growth regulator-free MS liquid medium (95.25%) as compared to the solid medium (89.00%). Shoot<br \/>cultures remained viable for up to 15 days without refreshing the medium and could be sustained for 12<br \/>months when sub-cultured every 15 days. Invigoration of albino shoots with fresh MS basal medium resulted<br \/>in multiple green buds, which successfully transformed into 54.60 and 652.78 healthy shoots in 30 and 120<br \/>days, respectively. These shoots were rooted, acclimatized, and transferred in field conditions. The present<br \/>study thus establishes a simple in vitro liquid culture system for the mass propagation of P. minima, providing<br \/>a strategy for the long-term conservation of multiple endangered plant species.<\/p>\r\n<figure id=\"attachment_2205\" aria-describedby=\"caption-attachment-2205\" style=\"width: 255px\" class=\"wp-caption alignnone\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-2205 size-medium\" src=\"http:\/\/ppsp.com.pk\/wp-content\/uploads\/2023\/07\/shoot-regenration-255x300.jpg\" alt=\"Different stages of in vitro shoot regeneration of Physalis minima \" width=\"255\" height=\"300\" srcset=\"https:\/\/ppsp.com.pk\/wp-content\/uploads\/2023\/07\/shoot-regenration-255x300.jpg 255w, https:\/\/ppsp.com.pk\/wp-content\/uploads\/2023\/07\/shoot-regenration-871x1024.jpg 871w, https:\/\/ppsp.com.pk\/wp-content\/uploads\/2023\/07\/shoot-regenration-768x903.jpg 768w, https:\/\/ppsp.com.pk\/wp-content\/uploads\/2023\/07\/shoot-regenration-1306x1536.jpg 1306w, https:\/\/ppsp.com.pk\/wp-content\/uploads\/2023\/07\/shoot-regenration.jpg 1527w\" sizes=\"(max-width: 255px) 100vw, 255px\" \/><figcaption id=\"caption-attachment-2205\" class=\"wp-caption-text\">Copyright Published material<\/figcaption><\/figure>\r\n\r\n<p>&nbsp;<\/p>\r\n\r\n<p class=\"wp-block-paragraph\" id=\"viewer-aoltd\">\u00a0<\/p>\r\n","protected":false},"excerpt":{"rendered":"<p>Saving the endangered flora of Physalis minima by plant tissue culture\u00a0 Physalis minima is a promising medicinal plant of the family Solanaceae, but its survival is underthreat, and limited long-term conservation approaches are available. We employed an in vitro plant tissueculture technique to develop a mass propagation system for the effective multiplication and conservation of&#8230;<\/p>\n","protected":false},"author":1,"featured_media":2772,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-2048","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts\/2048","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2048"}],"version-history":[{"count":4,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts\/2048\/revisions"}],"predecessor-version":[{"id":2206,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts\/2048\/revisions\/2206"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/media\/2772"}],"wp:attachment":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2048"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2048"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2048"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}