{"id":1,"date":"2023-06-23T16:44:51","date_gmt":"2023-06-23T16:44:51","guid":{"rendered":"https:\/\/ppsp.com.pk\/?p=1"},"modified":"2026-08-01T10:45:25","modified_gmt":"2026-08-01T10:45:25","slug":"hello-world","status":"publish","type":"post","link":"https:\/\/ppsp.com.pk\/?p=1","title":{"rendered":"In vitro flowering"},"content":{"rendered":"<p><a href=\"https:\/\/www.hindawi.com\/journals\/btri\/2014\/264690\/\">https:\/\/www.hindawi.com\/journals\/btri\/2014\/264690\/<\/a><\/p>\n<p><i>In vitro<\/i>\u00a0flowering and effective micropropagation protocol were studied in\u00a0<i>Swertia chirayita<\/i>, an important medicinal plant using axillary bud explants. The Murashige and Skoog&#8217;s medium (MS) supplemented with benzyl amino purine (BAP) 1.0\u2009\u00a0and adenine sulfate 70.0\u2009\u00a0was found optimum for production of multiple shoots. In the present study, incubation of flowering cultures on BAP supplemented medium (during shoot multiplication) was found necessary for flowering (6 weeks). However, concentrations of auxins-like IBA (0\u20132.0\u2009mg\/L) were ineffective to form reproductive buds. Subculture duration, photoperiod, and carbon source type do have influence on the\u00a0<i>in vitro<\/i>\u00a0flowering. The mature purple flowers were observed when the cultures were maintained in the same medium. This is the very first report that describes\u00a0<i>in vitro<\/i>\u00a0flowering system to overcome problems associated with flower growth and development as well as lay foundation for fruit and seed production\u00a0<i>in vitro<\/i>\u00a0in\u00a0<i>Swertia chirayita<\/i>.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>https:\/\/www.hindawi.com\/journals\/btri\/2014\/264690\/ In vitro\u00a0flowering and effective micropropagation protocol were studied in\u00a0Swertia chirayita, an important medicinal plant using axillary bud explants. The Murashige and Skoog&#8217;s medium (MS) supplemented with benzyl amino purine (BAP) 1.0\u2009\u00a0and adenine sulfate 70.0\u2009\u00a0was found optimum for production of multiple shoots. In the present study, incubation of flowering cultures on BAP supplemented medium (during [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":2767,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_eb_attr":"","_themeisle_gutenberg_block_has_review":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts\/1","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1"}],"version-history":[{"count":4,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts\/1\/revisions"}],"predecessor-version":[{"id":2242,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/posts\/1\/revisions\/2242"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=\/wp\/v2\/media\/2767"}],"wp:attachment":[{"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ppsp.com.pk\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}